Scope and Limitations

With the interest of determining which of the developmental stages is the most effective in culturing bacteria, the study makes use of the golden apple snails of three different stages- eggs, hatchlings, and adults. For sampling, the researchers consider the use of artificial culture to grow the snails for the purpose of maintaining objectivity and to make sure no external contaminants.  

Using the biuret protein assay it would determine the specific amount of protein. This in turn would be used in interpreting whether there is a correlation between golden apple snail developmental stages to their efficiency in growing Escherichia coli.

            To solely focus on the stages, the research eliminated aestivation as a factor to consider.