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E.coli Metabolism Products on Different Culture Mediums
In book source, In Escherichia coli, In original06 October 2011
E. coli typically produces positive tests for indole, lysine decarboxylase, and mannitol fermentation and produces gas from glucose. an isolate from urine can be quickly identified as E. coli by its hemolysis on blood agar typical colonial morphology with an iridescent "sheen" on different media such as EMB agar, and a positive spot indole test.Lactose fermented rapidly E. coli: Metallic sheen on different media: motile: flat: nonvicous...
Selective and differential agar
In book source, In culture medium, In original06 October 2011
EMB (Eosin Methylene Blue) agar inhibits gram positive bacteria, so it is instrumental in isolating gram negative pathogens of the lower digestive tract. the normal flora bacterium E.coli is readily detected on EMB agar by the green sheen that the colonies develop. most pathogens don't develop such as sheen, so microbiologists has a much easier job of isolating the organism responsible for the disease. EMB therefore both a selective and...
Bacterial culture Media
In culture medium, In internet source, In original06 October 2011
One of the most important reasons for culturing bacteria in vitro is its utility in diagnosinginfectious diseases. Isolating a bacterium from sites in body normally known to be sterile is anindication of its role in the disease process. Culturing bacteria is also the initial step in studyingits morphology and its identification. Bacteria have to be cultured in order to obtain antigensfrom developing serological assays or vaccines. Certain...
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